DNA Fingerprinting

The objective of this experiment is to learn how agarose gel electrophoresis separates different sizes of DNA fragments. Students will learn how these fragments form unique patterns for each person’s DNA, which is the basis for DNA fingerprinting analysis. As a prerequisite, students will learn to a) prepare a gel and b) how to load a gel.

No human or animal blood or body fluid is used in this experiment. Components for 6 experiments.

Components:

• Complete instructions, background information, and study questions
• Quick Strip Ready-to-Load Dye Samples
  • Crime Scene Simulated DNA Sample 1
  • Crime Scene Simulated DNA Sample 2
  • Suspect 1 Simulated DNA Sample 1
  • Suspect 1 Simulated DNA Sample 2
  • Suspect 2 Simulated DNA Sample 1
  • Suspect 2 Simulated DNA Sample 2
• UltraSpec-Agarose Powder
• Practice Gel Loading Solution
• 50x Electrophoresis Buffer
• Calibrated Transfer Pipets
• Graduated Cylinder
• Micropipets

Requirements:

• Horizontal Gel Electrophoresis Apparatus
• D.C. Power Supply
• Fixed micropipet with tips (optional)
• Microwave
• 250ml Flasks beaker or jar (8 oz.)
• Hot gloves and safety goggles
• Latex or vinyl gloves
• Distilled or Deionized water
• Measuring Spoons (1/8 and 1/4)
• 500 ml beaker or jar

Time Requirements:
Requires 45 minutes

Price: $45
Catalog # DD-132

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